Investigation of Oxidative Stress Status in Metabolic Syndrome Patients Using Lipid Peroxidation Biomarkers

Authors

  • Muhamed T Osman National Defence University of Malaysia (UPNM)
  • Rahman T
  • Ismail TS
  • Azlina A.R.
  • H. Nawawi

DOI:

https://doi.org/10.3823/1879

Keywords:

Metabolic syndrome, Oxidative test, Malondialdehyde, oxidized low density lipoprotein.

Abstract

Abstract                                                                         

          Metabolic syndrome (MS) is a cluster of interrelated common clinical disorders, including obesity, insulin resistance, glucose intolerance, hypertension and dyslipidaemia. Elevatedoxidative stress has been suggested as the underlying causes in the development of MS. The aim of this study was to determine the relationship between oxidative stress and metabolic syndrome (MS) among Malaysians and to assess the oxidative stress status in the syndrome using lipid peroxidation biomarkers. This cross-sectional study involved a total of 260 subjects (Mean±SD: 53±11 years, 66 Males) were randomly recruited and divided into five groups consisting of 52 patients for each group which are MS with newly diagnosed Diabetes Mellitus (MSDM), MS with Impaired Fasting Glucose (MSIFG), normoglycemia with MS (MSNG), central obesity without MS (OBXMS) and healthy subjects as a control group (NC).  Serum samples were analysed for malondialdehyde (MDA) and oxidized low density lipoprotein (Ox LDL). Detailed history taking and physical examination is performed to obtain relevant clinical data. Fasting blood samples of all subjects were collected, separated, stored and biochemical analysis were done to determine the levels of oxidative stress biomarkers in serum. Comparison of MDA levels between MS patients and NC showed that among the other 3 MS groups, MSDM had significantly higher level compared to NC (p<0.0001) and OB XMS (p<0.005). For Ox LDL, MSIFG had significantly higher level compared to NC and OBXMS (p<0.0001). In conclusion, this study clearly indicates that oxidative stress is the major contributor of Malaysian patients with metabolic syndrome with the evidence of increased level of lipid peroxidation biomarkers in human serum.

Author Biography

Muhamed T Osman, National Defence University of Malaysia (UPNM)

Department of Pathology, Faculty of Medicine and Defence Health

References

References

Moller DE and Kaufman KD. Metabolic syndrome: A clinical and molecular perspective. Annu. Rev. Med 2005; 56:45-62.

Alberti G, Zimmet P, Shaw J, Grundy SM. The IDF consensus worldwide definition of the metabolic syndrome. International Diabetes Foundation 2006 ; 1 :23.

Jiri Skalicky, Vladimira Muzakova, Roman Kandar, Milan Meloun, Tomas Rousar and Vladimir Palicka. Evaluation of oxidative stress and inflammation in obese adults with metabolic syndrome. Clin Chem Lab Med 2008; 46(4):499–505

Mustafa zbek, Evrim akır, Enis zkaya. Oxidative Stress Markers in Metabolic Syndrome. Turk Jem 2011; 15: 57-61

Betteridge DJ. What is the oxidative stress? Metabolism 2000; 4913-18.

Holvoet P, Lee D.H, Steffes M, Gross M, Jacobs DR Jr. Association between circulating oxidized low-density lipoprotein and incidence of the metabolic syndrome. JAMA 2008; 299: 2287-93.

Goldstein, B. J. and R. Scalia. Adiponectin: A Novel Adipokine Linking Adipocytes and Vascular Function. J Clin Endocrinol Metab 2004 ; 89(6): 2563-2568.

Montuschi, P., P. J. Barnes. Isoprostanes: markers and mediators of oxidative stress." FASEB J 2004 ; 18(15): 1791-1800.

Usta M, Turan E, Aral H, Inal BB, Gurel MS, Guvenen G. Serum paraoxonase-1 activities and oxidative status in patients with plaquetype psoriasis with/without metabolic syndrome. J Clin Lab Anal 2011; 25:289-95.

Redon, J., M. R. Oliva. Antioxidant Activities and Oxidative Stress Byproducts in Human Hypertension. Hypertension 2003; 41(5): 1096-1101.

Kand’a´ r R, Zˇ a´ kova´ P, Muzˇa´kova´ V. Monitoring of antioxidant properties of uric acid in humans for a consideration measuring of levels of allantoin in plasma by liquid chromatography. Clin Chim Acta 2006; 365:249–56.

Sjogren, P., S. Basu. "Measures of Oxidized Low-Density Lipoprotein and Oxidative Stress Are Not Related and Not Elevated in Otherwise Healthy Men With the Metabolic Syndrome." Arterioscler Thromb Vasc Biol 2005; 25(12): 2580-2586.

Wallenfeldt, K., B. Fagerberg. Oxidized low-density lipoprotein in plasma is a prognostic marker of subclinical atherosclerosis development in clinically healthy men. Journal of Internal Medicine 2004; 256(5): 413-420.

Zimmet, P., K. Alberti. A New International Diabetes Federation (IDF) Worldwide Definition of the Metabolic Syndrome: the Rationale and the Results." Revista Española de Cardiología (Internet) 2005 ; 58(12): 1371-1375.

Holvoet, P., J. Vanhaecke. Oxidized LDL and Malondialdehyde-Modified LDL in Patients With Acute Coronary Syndromes and Stable Coronary Artery Disease. Circulation 1998 ; 98(15): 1487-1494.

Ledwozyw, A., Michalak, J., Stepien A. and Kadziolka, A. The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clin. Chim. Acta 1986; 155:275-284.

S. I. Toshima, A. Hasegawa,M. Kurabayashi. Circulating oxidized low density lipoprotein levels: a biochemical risk marker for coronary heart disease, Arteriosclerosis, Thrombosis, and Vascular Biology 2000; 20:2243–2247.

P. Libby, P. M. Ridker, and A. Maseri. Inflammation and atherosclerosis, Circulation 2002; 105:1135–1143.

B. Hansel, P. Giral, E. Nobecourt. Metabolic syndrome is associated with elevated oxidative stress and dysfunctional dense high-density lipoprotein particles displaying impaired antioxidative activity. Journal of Clinical Endocrinology and Metabolism 2004; 89: 4963–4971.

M. Jacobs, M. M. J. Van Greevenbroek, C. J. H. Van Der Kallen. Low-grade inflammation can partly explain the association between the metabolic syndrome and either coronary artery disease or severity of peripheral arterial disease: the CODAM study. European Journal of Clinical Investigation 2009; 39-6: 437–444.

J.-P. Despr´es. Inflammation and cardiovascular disease: is abdominal obesity the missing link?. International Journal of Obesity 2003; 27-3: S22–S24.

H. M. Lakka, D. E. Laaksonen, T. A. Lakka. The metabolic syndrome and total and cardiovascular disease mortality in middle-aged men. Journal of the American Medical Association 2002; 288- 21: 2709–2716.

Valle Gottlieb MG, da Cruz IB, Duarte MM. Associations among Metabolic Syndrome, Ischemia, Inflammatory, Oxidatives, and Lipids Biomarkers. J Clin Endocrinol Metab 2010; 95:586-91.

Duarte MM, Rocha JB, Moresco RN. Association between ischemia-modified albumin, lipids and inflammation biomarkers in patients with hypercholesterolemia. Clin Biochem 2009; 42:666-71

Janssen, I., P. T. Katzmarzyk. Waist circumference and not body mass index explains obesity-related health risk. American Journal of Clinical Nutrition 2004; 79: 379-384.

Alexander, C. M., Landsman, P. B., Teutsch, S. M., Haffner, SM. NCEP-defined metabolic syndrome, diabetes, and prevalence of coronary heart disease among NHANES III participants age 50 years and older. Diabetes 2003; 52: 1210–1214.

Alenezi, M. Y., M. Marcil. Is the Decreased High-Density Lipoprotein Cholesterol in the Metabolic Syndrome Due to Cellular Lipid Efflux Defect? J. Clin Endocrinol Metab 2004 ; 89(2): 761-764.

Brasier AR, Resinos A III, Eledrisi MS. Vascular inflammation and the renin-angiotensin system. Arterioscler Thromb Vasc Biol 2002 ; 22:1257–66.

Knopp RH, Paramsothy P. Oxidized LDL and abdominal obesity: a key to understanding the metabolic syndrome. Am J Clin Nutr 2006 ; 83:1–2.

Hansel B, Giral P, Nobecourt E, Chantepie S, Bruckert E, Chapman J. Metabolic syndrome is associated with elevated oxidative stress and dysfunctional dense highdensity lipoprotein particles displaying impaired antioxidative activity. J Clin Endocrinol Metab 2004 ; 89:4963– 71.

Furukawa, S., T. Fujita. Increased oxidative stress in obesity and its impact on metabolic syndrome. Journal of Clinical Investigation 2004 ; 114(12): 1752.

Holvoet, P., S. B. Kritchevsky. The Metabolic Syndrome, Circulating Oxidized LDL, and Risk of Myocardial Infarction in Well-Functioning Elderly People in the Health, Aging, and Body Composition Cohort. Diabetes 2004; 53(4): 1068-1073.

Veena S. Rao, Radhika K. Nagaraj, Sridhara Hebbagodi, Natesha B. Kadarinarasimhiah, and Vijay V. Kakkar. Association of Inflammatory and Oxidative StressMarkers with Metabolic Syndrome in Asian Indians in India. Cardiology Research and Practice 2011 ; 10: 4061-69.

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Published

2016-02-04

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Section

Laboratory Medicine