The antimicrobial potential of Lactobacillus acidophilus on pathogenic bacteria causing diarrhea
Keywords:Probiotic, L. acidophilus, Bacterial Diarrhea
Object: Lactobacillus acidophilus is a nonpathogenic member of gastrointestinal tract and it is widely used in fermented dairy products.Â This study aimed to assess the antimicrobial potential of two strainsÂ of L. acidophilus on some pathogenic bacteria frequently causing diarrhea or gastroenteritis.
Methods: The antibacterial activity cell free supernatant (CFS) of twoÂ control standard strains of L. acidophilus (L. acidophilus-la5 and L.Â acidophilus against fie control standard strains of bacteria causingÂ diarrhea; Enterotoxogenic Escherichia coli (ETEC), EnterohaemorrhagicÂ E. coli OÂ 157:H7(EHEC) Salmonella typhimurium Shigella flxeneri andÂ Staphylococcus aureus were determined using agar well diffusionÂ method. The sensitivity of the pathogenic bacteria to the CFS of eachÂ L. acidophilus in relation to time was determined by standard plateÂ count .The antibiotic susceptibility tests of 20 antibiotics against tested organisms with and without CFS were assessed by disc diffusionÂ method. The minimum inhibitory concentration (MIC) of ciproflxacinÂ with and without CFS was determined by tube dilution method.
Results: Both Lactobacilli strains decrease the colony count of testedÂ strains by more than 90% after 60 min contact time. Both LactobacilliÂ strains signifiantly improve the antibacterial effect of tested antibiotics against ETEC, S. typhimurium and S. aureus, and S. flxeneri (P<Â 0.05). The MIC of ciproflxacin alone against all tested strains wasÂ 15.625 Î¼g/ml, while when combined with both Lactobacilli CFSs, theÂ MIC decreased signifiantly to 0.488 Î¼g/ml for ETEC, S. typhimurium,Â and S. flxeneri and to 0.977 Î¼g/ml for EHEC and S. aureus (P= 0.000).
Conclusion: living L. acidophilus strains could be used in preventionÂ and treatment of diarrhea caused by certain bacterial pathogens, either in fermented milk/ yoghurt or as mediations.
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