In Vitro and in Vivo Antineoplastic Activity of Barbatic Acid

  • Mônica Cristina Barroso Martins UNIVERSIDADE FEDERAL DE PERNAMBUCO
  • Tamiris Alves Rocha
  • Thiago David Santos Silva
  • Marinaldo Pacífico Cavalcanti-Neto
  • Noemia Pereira da Silva Santos
  • Teresinha Gonçalves da Silva
  • Francisco Carlos Amanajás Aguiar-Junior
  • Emerson Peter da Silva Falcão
  • Eugênia C Pereira
  • Nicácio Henrique da Silva


Background: Lichen compounds exhibit remarkable biological activity. Several of them are potent anticancer agent and some with low or none toxicity. The aim of this study was perform in vitro and in vivo assessments of the antineoplastic activity of organic extract and barbatic acid (BAR) isolated from the lichen Cladia aggregata (Sw.) Nyl.

 Methods: In vitro assays were performed with both extract and BAR against HEp-2 (Adenocarcinoma of the Larynx), NCI-H292 (Squamous Cell Lung Carcinoma) and KB (Nasopharyngeal Squamous Cell Carcinoma) cells. The tests were carried out on the Sarcoma-180 BAR, tumor and organs were analyzed histopatologically after 7 days of chemotherapy.

 Results: Cytotoxic tests with BAR revealed 50% inhibitory concentration (IC50) of 19.06 µg mL-1 for NCI-H292 and 12.0 µg mL-1 for KB and 6.25 µg mL-1 for HEp-2 cells. Tests with Sarcoma-180 demonstrated 46.3% inhibitory activity against the tumor by BAR. This substance showed no significant effect on the expression of argyrophilic nucleolar organizer regions proteins (AgNORs). The histopathology study of neoplastic tissue, demonstrated that cell proliferation was not affected by the antineoplastic action of the compound tested.

 Conclusions: The results indicate that barbatic acid exhibits significant antineoplastic activity and low toxicity rate.

May 30, 2016
How to Cite
BARROSO MARTINS, Mônica Cristina et al. In Vitro and in Vivo Antineoplastic Activity of Barbatic Acid. International Archives of Medicine, [S.l.], v. 9, may 2016. ISSN 1755-7682. Available at: <>. Date accessed: 07 may 2021. doi:
Laboratory Medicine


Antitumor, Citotoxity activity, Depside, Lichen, Lichen substance, Natural Compound