Inducible Nitric Oxide Synthase in the Placenta of Pregnant Women with Preeclampsia

Authors

  • Juan Carlos Medrano-Rodríguez UNIVERSIDAD AUTÓNOMA DE ZACATECAS
  • Eduardo Medrano-Cortés
  • Christian Starlight Franco-Trejo
  • Hilda Saray Contreras de la Fuente

DOI:

https://doi.org/10.3823/1819

Keywords:

Inducible Nitric Oxide Synthase, Placenta, Pre-Eclampsia

Abstract

Preeclampsia (PEC) is the cause of fetal and maternal death. Inducible nitric oxide synthase (iNOS) found in macrophages, smooth muscle cells and endothelial cells. The main objective was to detect the fluorescence iNOS in placenta. Cotyledons cuts were made, they were placed in 10% formalin. And on the slides washed with buffer solution, oxygenated water with methanol, using 10% pig serum, anti iNOS Rabbit pAb solution, streptavidin-fluorescein. Microscope Carl Zeiss
and Image-Pro 7.0 software was used. Placentas of pregnant women with PEC without pathology were analyzed. Increased uptake of fluorescein in erythrocytes of women without PEC was found, patients who developed PEC was remarkable decreased uptake of fluorescein. Decreasing iNOS activity is evident in preeclampsia.

Author Biography

Juan Carlos Medrano-Rodríguez, UNIVERSIDAD AUTÓNOMA DE ZACATECAS

Docente-Investigador Responsable del Doctorado en Ciencias en la Especialidad de Farmacología Médica y Molecular

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Published

2015-09-14

Issue

Section

International Online Biomedical Conference