Inducible Nitric Oxide Synthase in the Placenta of Pregnant Women with Preeclampsia
Keywords:Inducible Nitric Oxide Synthase, Placenta, Pre-Eclampsia
Preeclampsia (PEC) is the cause of fetal and maternal death. InducibleÂ nitric oxide synthase (iNOS) found in macrophages, smooth muscleÂ cells and endothelial cells. The main objective was to detect the fluorescenceÂ iNOS in placenta. Cotyledons cuts were made, they were placedÂ in 10% formalin. And on the slides washed with buffer solution,Â oxygenated water with methanol, using 10% pig serum, anti iNOSÂ Rabbit pAb solution, streptavidin-fluorescein. Microscope Carl Zeiss
and Image-Pro 7.0 software was used. Placentas of pregnant womenÂ with PEC without pathology were analyzed. Increased uptake of fluoresceinÂ in erythrocytes of women without PEC was found, patientsÂ who developed PEC was remarkable decreased uptake of fluorescein.Â Decreasing iNOS activity is evident in preeclampsia.
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